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. 2018 Mar 22;15(5):7639–7648. doi: 10.3892/ol.2018.8321

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Melting curves for (A) protocadherin γ subfamily B, 6, (B) O₆-methylguanine-DNA methyltransferase, (C) homeobox A9, (D) suppressor of cytokine signaling 3, (E) Ras association domain family member 5 and (F) microRNA-126 in the methylation-sensitive high-resolution melting assay. Artificially prepared methylation gradient ratios were used to evaluate the methylation index of each sample. The horizontal axis indicates different melting temperatures of gradient-dilution standard DNA samples and vertical axis indicates the corresponding slopes. Methylated DNA that contains cytosines has an increased melting temperature than unmethylated DNA.