Figure 1.

Met inhibits the proliferation of OS cells. Cell Counting kit-8 analysis was utilized to evaluate the proliferation of (A) MG63 and (B) U2OS OS cells treated with the indicated concentrations of Met for 0, 24 and 48 h. (C) MG63 and (D) U2OS cells were plated in 6-well plates at a density of 1,000 cells/well with the indicated concentrations of Met. At 2 weeks, the number of colonies was counted. *P<0.05, **P<0.01. Met, metformin; OS, osteosarcoma; Con, control (0 mM Met); OD, optical density.