Figure 2.

Met inhibits the migration and invasion of osteosarcoma cells. The wound area was measured prior to and at 48 h after the treatment of (A) MG63 and (B) U2OS cells with the indicated concentrations of Met (magnification, ×400). The number of invading (C) MG63 and (D) U2OS cells in Matrigel-coated Transwell inserts were counted at 24 h after plating (magnification, ×400). Representative images of western blotting results for epithelial-mesenchymal transition-associated markers, including E-cadherin and vimentin, in (E) MG63 and (F) U2OS cells following the indicated treatments are presented. *P<0.05, **P<0.01. Met, metformin; Con, control (0 mM Met).