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Transfection efficiency was confirmed by RT-qPCR and western blot analysis. The (A) mRNA and (B) protein levels of TFAP4 were increased following overexpression of TFAP4 in SW480 cells. (C) RT-qPCR and (D) western blot analysis revealed that si-002 was the most efficient siRNA for TFAP4 inhibition. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; si, small interfering; TFAP4, transcription factor AP4.
