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. 2018 Mar 8;15(5):6967–6974. doi: 10.3892/ol.2018.8211

Figure 3.

Figure 3.

Transfected siRNA into 786-0 and GRC-1 renal cancer cells targets the expression of ACLY at both mRNA and protein level. (A) Relative expression of ACLY mRNA in quantified by real-time PCR in 786-0, GRC-1, 786-0 cells transfected with negative control, 786-0 cells transfected with siRNA and HK-2 cells (**P<0.01 compared with the HK-2. #P<0.05 compared with 786-0 and GRC-1). (B) Differential expression ACLY protein levels illustrated by western blotting in above five cell lines. (C) Analysis of ACLY protein expression shows lower expression in transfected with siRNA than non-treated cells or treated cells with negative control (**P<0.01 compared with the HK-2. #P<0.05 compared with 786-0 and GRC-1). ACLY, ATP citrate lyase; RCC, renal cell carcinoma; siRNA, small interferring RNA.