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. 2018 Mar 8;15(5):6967–6974. doi: 10.3892/ol.2018.8211

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Copyright: © Teng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — ACLY siRNA promotes apoptosis of RCC cells. Inhibition of ACLY by transfected siRNA promoted apoptosis of 786-0 cells and HK-2 cells. The upper panel is the typical flow cytometric images, and lower panel is the result of analysis (**P<0.01 compared with the HK-2. ^#P<0.05 compared with 786-0 and GRC-1). Typical images of apoptosis in 786-0, GRC-1, 786-0 cells transfected with negative control, 786-0 cells transfected with siRNA and HK-2 cells are displayed by flow cytometry analysis. The percentage of apoptotic RCC cells shows a significantly increase after transfection of siRNA (**P<0.01 compared with the HK-2. ^#P<0.05 compared with 786-0 and GRC-1). ACLY, ATP citrate lyase; RCC, renal cell carcinoma; siRNA, small interferring RNA.