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. 2018 Mar 7;15(5):7291–7296. doi: 10.3892/ol.2018.8200

Figure 2.

Figure 2.

Downregulation of HOXC8 decreases cervical cancer cell proliferation in vitro. (A) Downregulation of HOXC8 in cervical cancer cell lines (achieved by using HOXC8-specific siRNA) was confirmed using RT-qPCR. (B) Downregulation of HOXC8 in SiHa cell line (achieved by using HOXC8-specific siRNA) was confirmed using western blot analysis. (C) Downregulation of HOXC8 in HT-3 cell line (achieved by using HOXC8-specific siRNA) was confirmed using western blot analysis. (D) Cell Counting Kit-8 assay was used to determine the cell proliferation of cervical cancer cell lines (SiHa and HT-3) and normal cervical cell line (Crl-2614). (E) Cell Counting Kit-8 assay was used to determine the cell proliferation of cervical cancer cell line SiHa following transfection with HOXC8-specific and negative control-siRNAs. (F) Cell Counting Kit-8 assay was used to determine the cell proliferation of cervical cancer cell line HT-3 following transfection with HOXC8-specific and negative control-siRNAs. *P<0.05, **P<0.01, ***P<0.001. HOXC8, homeobox C8; siRNA, small-interfering RNA; OD, optical density; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.