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. 2018 Mar 1;15(5):6611–6621. doi: 10.3892/ol.2018.8140

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Figure 1. — CD133⁺ ovarian cancer cells have ovarian cancer stem cell properties. (A) The mRNA expression levels in OVCAR-3 ovarian cancer cells were analyzed by RT-qPCR (B) CD133⁺ OVCAR-3 ovarian cancer cells generated more tumor spheres than CD133⁻ OVCAR-3 ovarian cancer cells. (C) The mRNA expression levels in PA-1 ovarian cancer cells were analyzed by RT-qPCR (D) CD133⁺ PA-1 ovarian cancer cells generated more tumor spheres than CD133⁻ PA-1 ovarian cancer cells. Data are expressed as the mean ± SD (n=3). Tumor growth curves for (E) OVCAR-3 and (F) PA-1 ovarian cancer cells. CD133⁺ or CD133⁻ ovarian cancer cells (5×10⁵) were inoculated in BALB/c nude mice. The data are expressed as the mean ± SD (n=6). All data were analyzed using independent samples Student's t-tests. *P<0.05, **P<0.01 and ***P<0.001 vs. CD133⁻. CD, cluster of differentiation; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; SD, standard deviation.