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. 2018 Mar 6;15(5):4325–4331. doi: 10.3892/etm.2018.5929

Figure 1.

Figure 1.

miR-223 regulates IGF-1R expression in the Hep3B cell line. (A) The 224–231 of the IGF-1R 3′-untranslated region is the putative target site of miR-223. (B) Relative mRNA expression of IGF-1R in cells transfected with miR-223 mimic, miR-223 inhibitor and NC, compared with the BL. The expression of IGF-1R mRNA was normalized to GAPDH. (C) Expression of IGF-1R protein detected by western blotting in cells transfected with miR-223 mimic, miR-223 inhibitor or NC, compared with the BL group. GAPDH was used as a loading control. Quantified data are presented as the mean ± standard deviation. **P<0.01 vs. BL. BL, blank control; NC, negative control RNA; mimic, miR-223 mimic; inhibitor, miR-223 inhibitor; IGF-1R, insulin-like growth factor-1 receptor; miR-223, microRNA-223.