Table 1.

Substrate Specificity of SpNanC

Substrate	Percentage conversion (%)
Neu5Acα2–3GalβpNP	85.1 ± 2.4
Neu5AcFα2–3GalβpNP	74.8 ± 3.2
Neu5AcOMeα2–3GalβpNP	81.8 ± 1.7
Neu5AcN3α2–3GalβpNP	87.9 ± 1.6
Neu5Ac9Fα2–3GalβpNP	75.6 ± 3.9
Neu5Ac9OMeα2–3GalβpNP	88.5 ± 5.1
Neu5Ac9deoxyα2–3GalβpNP	93.5 ± 3.1
Neu5Ac7Fα2–3GalβpNP	91.0 ± 4.3
Neu5Ac7OMeα2–3GalβpNP	96.5 ± 1.7
Neu5Ac7deoxyα2–3GalβpNP	91.2 ± 1.8
Neu5Ac7N3α2–3GalβpNP	65.9 ± 1.1
Neu5Gcα2–3GalβpNP	80.6 ± 6.7
Neu5Gc9Fα2–3GalβpNP	72.3 ± 2.6
Neu5Gc9OMeα2–3GalβpNP	75.0 ± 1.9
Neu5Gc9deoxyα2–3GalβpNP	85.5 ± 1.4
Neu5Gc9N3α2–3GalβpNP	89.5 ± 3.5
Kdnα2–3GalβpNP	4.50 ± 0.45
Kdn5Fα2–3GalβpNP	0.48 ± 0.00
Kdn5N3α2–3GalβpNP	0.32 ± 0.45
Kdn5deoxyα2–3GalβpNP	5.31 ± 0.23
Kdn5OMeα2–3GalβpNP	1.53 ± 1.02
Kdn9Fα2–3GalβpNP	3.70 ± 1.14
Kdn9OMeα2–3GalβpNP	4.02 ± 1.36
Kdn7Fα2–3GalβpNP	0.56 ± 0.11
Kdn7OMeα2–3GalβpNP	23.4 ± 3.3
Kdn7deoxyα2–3GalβpNP	10.1 ± 0.0
Kdn7N3α2–3GalβpNP	4.50 ± 0.45