Extended Data Table 1.
Statistics of data collection and refinement
| Apo pH7.0 | Agonist-bound | |
|---|---|---|
| Data Collection | ||
| Microscope | Titan Krios (FEI) | Titan Krios (FEI) |
| Voltage (kV) | 300 | 300 |
| Detector | K2 Summit (Gatan) |
K2 Summit (Gatan) |
| Pixel size (Å) | 1.0 | 1.0 |
| Defocus range (μm) | −1.0 to −3.0 | −1.0 to −2.5 |
| Movies | 1172 | 1179 |
| Frames/movie | 50 | 50 |
| Dose rate (electrons/pixel/s) | 8 | 8 |
| Total dose (electrons/Å2) | 80 | 80 |
| Number of particle | 111762 | 201368 |
|
| ||
| Model composition | ||
| Non-hydrogen atoms | 14640 | 15538 |
| Protein residues | 1828 | 1920 |
| Ligand | 0 | 4 |
| Refinement | ||
| Number of particle for refinement | 56105 | 63111 |
| Resolution (Å) | 3.72 | 3.49 |
| RMS deviations | ||
| Bond lengths (Å) | 0.0085 | 0.0077 |
| Bond angles (°) | 1.2531 | 1.1373 |
| Ramachandran plot (%) | ||
| Favored | 90.73 | 90.63 |
| Allowed | 8.99 | 9.11 |
| Disallowed | 0.28 | 0.26 |
| Clashscore, all atoms | 4.42 | 3.55 |
| Molprobity score | 2.10 | 1.87 |