Table I.
Primers used for reverse transcription-quantitative polymerase chain reaction.
| Target genes | NCBI Gene ID | Direction | Sequence (5′-3′) |
|---|---|---|---|
| NKX2-1 | 7080 | Forward | AGCACACGACTCCGTTCTC |
| Reverse | GCCCACTTTCTTGTAGCTTTCC | ||
| MSTN | 2660 | Forward | GACGATTATCACGCTACAACGG |
| Reverse | TCCATAGTTGGGCCTTTACTACT | ||
| CCR4 | 1233 | Forward | AGAAGGCATCAAGGCATTTGG |
| Reverse | ACACATCAGTCATGGACCTGAG | ||
| PRTN3 | 5657 | Forward | AACTACGACGCGGAGAACAAA |
| Reverse | CGAGGGACGAAAGTGCAAATG | ||
| TREH | 11181 | Forward | TGGGCTTGCTCATTCAGTCA |
| Reverse | TCCCCGTGGCAGTAAATCTC | ||
| GHR | 2690 | Forward | CCATTGCCCTCAACTGGACTT |
| Reverse | AATATCTGCATTGCGTGGTGC | ||
| NR1I2 | 8856 | Forward | GCCCATGCTGAAATTCCACTA |
| Reverse | GCCGATTGCATTCAATGTAGGA | ||
| PROM1 | 8842 | Forward | AGTCGGAAACTGGCAGATAGC |
| Reverse | GGTAGTGTTGTACTGGGCCAAT | ||
| PPARGC1A | 10891 | Forward | TGAAGACGGATTGCCCTCATT |
| Reverse | GCTGGTGCCAGTAAGAGCTT | ||
| CD40LG | 959 | Forward | GAGCAACAACTTGGTAACCCT |
| Reverse | GGCTGGCTATAAATGGAGCTTG | ||
| PI3 | 5266 | Forward | CACGGGAGTTCCTGTTAAAGG |
| Reverse | TCTTTCAAGCAGCGGTTAGGG | ||
| KIT | 3815 | Forward | TGCTCTGCTTCTGTACTGCC |
| Reverse | GCCTTACATTCAACCGTGCC | ||
| MYC | 4609 | Forward | CATCAGCACAACTACGCAGC |
| Reverse | GCTGGTGCATTTTCGGTTGT | ||
| JUN | 3725 | Forward | GTGCCGAAAAAGGAAGCTGG |
| Reverse | CTGCGTTAGCATGAGTTGGC | ||
| GAPDH | 2597 | Forward | GGAGCGAGATCCCTCCAAAAT |
| Reverse | GGCTGTTGTCATACTTCTCATGG | ||
| U6 | 26827 | Forward | AATTGGAACGATACAGAGAAGATT |
| Reverse | TATGGAACGCTTCACGAATTTG |
NCBI, National Center for Biotechnology Information; NKX2-1, NK2 homeobox 1; MSTN, myostatin; CCR4, C-C motif chemokine receptor 4; PRTN3, proteinase 3; TREH, trehalase; GHR, growth hormone receptor; NR1I2, nuclear receptor subfamily 1 group I member 2; PROM1, prominin 1; PPARGC1A, PPARG coactivator 1 α; CD40LG, CD40 ligand; PI3, peptidase inhibitor 3; KIT, KIT proto-oncogene receptor tyrosine kinase; MYC, MYC proto-oncogene, bHLH transcription factor; JUN, Jun proto-oncogene, AP-1 transcription factor subunit; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.