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. 2018 Mar 22;15(5):4441–4447. doi: 10.3892/etm.2018.5990

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Copyright: © Tong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Puerarin (Pue) treatment inhibits Th1 response. (A) Spleen lymphocytes were isolated and cultured in the presence of ConA (5 mg/ml). After 24 h, the supernatant was collected and the secretion of IFN-γ was detected by ELISA. (B) Spleen lymphocytes were obtained and cultured following stimulation with ovalbumin (OVA) (50 µg/ml). After 24 h, cell proliferation was conducted for MTT assay. *P<0.05 and **P<0.01, comparison with the delayed-type hypersensitivity (DTH) group. ^###P<0.001, comparison with the Control group. Dex, Dexamethasone.