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. 2018 Mar 22;15(5):4441–4447. doi: 10.3892/etm.2018.5990

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Copyright: © Tong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effect of puerarin (Pue) on Th1 and Th2 differentiation. Spleen lymphocytes were isolated and total RNA were extracted and quantitated. (A) Quantitative analysis of T-bet and (B) GATA-3 was handled, and (C) the ratio of T-bet/GATA-3 was calculated. (D) Quantitative analysis of IFN-γ and (E) IL-4 was handled and (F) the ratio of IFN-γ/IL-4 was calculated. *P<0.05 and **P<0.01, comparison with the delayed-type hypersensitivity (DTH) group. ^#P<0.05, ^##P<0.01 and ^###P<0.001, comparison with the Control group.