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. 2018 Apr 5;4(4):512–520. doi: 10.1021/acscentsci.8b00098

Figure 2.

Figure 2

Cargo identity alters relative CPP efficacy. (A) Each CPP sequence was analyzed for delivery of both a fluorophore (Cy5.5, MW = 665 Da) and a PMO (IVS2-654, MW = 6212 Da). For Cy5.5-CPP conjugates, HeLa-654 cells were treated with 5 μM of the conjugate in media containing 10% FBS. After 2 h, the Cy5.5 fluorescence was measured by flow cytometry. For PMO–CPP conjugates, HeLa-654 cells were treated with 5 μM of the conjugate in media containing 10% FBS. After 22 h, the eGFP fluorescence was measured by flow cytometry. (B) The cargo dependency of a given CPP, normalized to the activity of the highest-performing CPP for each type of cargo. There is little relationship between the CPPs that led to the most cellular Cy5.5 fluorescence and the CPPs that led to the most eGFP fluorescence.