Expression of ACO1, PSY1, and E4 in the fruit of transgenic and non-transformed plants (A). Total RNA (20 μg) was isolated from fruit at breaker stage from (lanes 1–6) non-transformed Nr, transformant 1223, transformant 1166, transformant 1193, transformant 1196, and non-transformed wild type (wt). Ethidium staining of RNA prior to blotting confirmed RNA loading (B).