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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2018 Mar 15;38(5):1125–1133. doi: 10.1161/ATVBAHA.118.310771

Figure 2.

Figure 2

Histologic analysis of ischemic limb in SS and AA mice 28 days after HLI. (A) Representative trichrome staining showed significant loss of muscle mass, tissue necrosis (blue staining) and excessive inflammation in SS mice compared to AA mice. Scale bar =40 μm. (B) Representative αSMA staining showed no difference between SS and AA. Scale bar = 200μm. (C) Representative Z-stack confocal microscopy of FITC-lectin perfused vessels showing significantly decreased functional vessel formation. Scale bar = 100 μm. Number of lectin-perfused vessel per mm2 is indicated. (D) Mice were injected with Evan’s blue dye, and spectrophotometric absorbance of harvested ischemic (IL) and non-ischemic (NIL) limbs was read at 620nm to evaluate vascular leakage. (E) Representative H&E of non-perfused blood vessel showing patency in the SS. N = 4–6 mice per genotype per indicated study. Data are plotted as mean ± SEM. *p<0.05, **p<0.01, ***p<0.001