Extended Data Table 5.
Mass spectromeric signals and calculated mass of photo-crosslinked Y1R with [Bpa1,K4[(Ahx)2-biotin]]NPY
| MALDIToF MS (m/z)* | Number in Y1R | Position [Bpa1,K4[(Ahx)2-biotin]]NPY | Mcalc.(Da)† | [Mcalc + (Da) | [Mcalc + Na]+(Da) | [Mcalc + K]+ (Da) |
|---|---|---|---|---|---|---|
| 1824.2 | not identified | |||||
| 1867.3 | not identified | |||||
| 1892.6 | 11–32 | 1 – 6 | 3760.7 | 3761.8 | 3783.7 | 3799.7 |
| 1988.2 | – | 1–11 + 5–6 | 1986.9 | 1987.9 | 2009.9 | 2025.9 |
| 2001.1 | – | 1–10 + 5–7 | 2000.0 | 2001.0 | 2022.9 | 2038.9 |
| 2059.2 | – | 1–10 + 8–11 | 2057.9 | 2058.9 | 2080.9 | 2096.9 |
| – | 1–11 +8–10 | 2057.9 | 2058.9 | 2080.9 | 2096.9 | |
| 2073.2 | not identified | |||||
| – | 1–10 + 8–11 | 2057.9 | 2058.9 | 2080.9 | 2096.9 | |
| 2081.3 | – | 1–11 +8–10 | 2057.9 | 2058.9 | 2080.9 | 2096.9 |
| 2121.3 | not identified | |||||
| 2162.2 | 191 – 194 | 1 – 10 | 2139.0 | 2140.0 | 2162.0 | 2178.0 |
| 2311.4 | not identified | |||||
| 2317.4 | not identified | |||||
| 2381.4 | 191 – 195 | 1–11 | 2380.1 | 2381.1 | 2403.1 | 2419.1 |
| 2381.8 | 195–205 | 1 – 4 | 2381.1 | 2382.1 | 2404.1 | 2420.1 |
| 2397.3 | 32–32 | 1 – 16 | 2374.0 | 2375.0 | 2397.0 | 2413.0 |
| 194–194 | 1 – 16 | 2374.0 | 2375.0 | 2397.0 | 2413.0 | |
| 21 – 21 | 1 – 16 | 2387.1 | 2388.1 | 2410.1 | 2426.1 | |
| 2410.3 | 195 – 195 | 1 – 16 | 2387.1 | 2388.1 | 2410.1 | 2426.1 |
| 194–195 | 1 – 15 | 2387.1 | 2388.1 | 2410.1 | 2426.1 | |
| 2413.3 | 32–32 | 1 – 16 | 2374.0 | 2375.0 | 2397.0 | 2413.0 |
| 194 – 194 | 1 – 16 | 2374.0 | 2375.0 | 2397.0 | 2413.0 | |
| 2450.4 | – | 1–15 + 8–10 | 2427.1 | 2428.1 | 2450.1 | 2466.1 |
| 2514.5 | not identified | |||||
| 2553.5 | 194 – 200 | 1 – 10 | 2530.1 | 2531.1 | 2553.1 | 2569.1 |
| 2589.6 | not identified | |||||
| 2775.7 | not identified | |||||
| 2807.7 | not identified | |||||
| 3031.7 | 22–29 | 1 – 15 | 3030.4 | 3031.4 | 3053.4 | 3069.4 |
| 3377.7 | 191 – 200 | 1 – 15 | 3376.5 | 3377.5 | 3399.5 | 3415.5 |
| 3784.1 | 11–32 | 1 – 6 | 3760.7 | 3761.8 | 3783.7 | 3799.7 |
| 3966.3 | – | 1–7 + 17–36 | 3943.1 | 3944.1 | 3966.1 | 3982.1 |
| 4524.9 | not identified |
*
Determined signals by MALDI-ToF MS.
†
Selected calculated masses in Dalton of possible photo-crosslinked fragments of Y1R with [Bpa1,K4[(Ahx)2-biotin]]NPY or [Bpa1,K4[(Ahx)2-biotin]]NPY with itself. The fragments are selected based on the correlation with the detected signals. For clarity, further calculated masses of possible photo-crosslinked fragments are not shown.