Table I.
High throughput screening of miRNAs with abnormal expression in MM.
| Type of sample | Sample size | Platform | Dysregulated miRs in MM validated by RT-qPCR | Refs. |
|---|---|---|---|---|
| CD138+ PCs, MM cell lines | 41 MM cell lines, 10 untreated MM, 4 N | Academic miRNA microarray | Up: miR-32, miR-17-5, miR-19a, miR-19b, miR-20a, miR-92, miR-106a, miR-106b, miR-93, miR-25, miR-181a/181b | (36) |
| CD138+ PCs, MM cell lines | 7 MM cell lines, 10 untreated MM, 2 N | miRCURY™LNA microarray | Up: miR-365, miR-193 | (37) |
| CD138+ PCs | 15 relapsed/refractory MM, 4 N | Liquid phase Luminex microbead miRNA profiling | Up: miR-181a/181b, miR-382, miR-222, miR-221 Down: miR-15a, miR-16 | (38) |
| CD138+ PCs | 52 untreated MM, 2 N | Agilent miRNA microarray V2 | Up: miR-15b, miR-16, miR-19b, miR-21, miR-22, miR-29c, let-7a, let-7d, let-7f | (10) |
| CD138+ PCs | 33 untreated MM,5 MGUS, 9 N | µRNA microarray | Up: miR-21, miR-16, miR-221, miR-200b, miR-19a, miR-342; Down: miR-15a | (39) |
| Serum | 14 untreated MM, 21 follow-up MM, 7 N | Agilent miRNA 15K array | Up: let-7b, miR-106a Down: miR-98, miR-16, let-7c, let-7i | (40) |
| Peripheral mononuclear cells | 5 untreated MM, 5 N | Applied Biosystems miProfile miRNA qPCR arrays | Up: miR-16-1, miR-21, miR-24-1, miR-28, miR-33a, miR-101-1, miR-124-1, miR-125a, miR-125b-1, miR-129-1, miR-139, miR-145, miR-149, miR-202, miR-212, miR-221, miR-410, miR-424, miR-1297 Down: miR-23a, miR-520e | (41) |
| Extracellular supernatant fluid of BM | 20 relapsed/refractory MM, 8 N | Agilent miRNA microarray v3 | Down: let-7a, let-7b, miR-15a, miR-16, miR-20a, miR-106b, miR-223 | (42) |
| Serum | 7 untreated MM, 5 N | miRCURY™LNA microarray | Up: miR-214-3p, miR-135b-5p, miR-4254, miR-3658 miR-33b; Down: miR-19a, miR-92a | (43) |
| Serum | 7 untreated MM, 5 N | miRCURY™LNA microarray | Up: miR-214, miR-135b, miR-132; Down: miR-92a | (35) |
miR/miRNA, microRNA; MM, multiple myeloma; MGUS, monoclonal gammopathy of undetermined significance; N, normal controls; BM, bone marrow; Up, upregulated; Down, downregulated; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; CD138, syndecan 1; PC, plasma cells; Refs., references.