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Detection of a nodule-specific DNA fragment by differential display. cDNAs synthesized along mRNAs isolated from either nodules, leaves, or roots of Chinese milk vetch were used as templates in PCR with a combination of A21 and A22 oligonucleotide primers (Bex). The PCR products were separated by agarose gel electrophoresis and compared with each other. Arrow indicates a 148-bp nodule-specific band (f32). Molecular markers are λ-DNA- digested with StyI.
