Alignment of the deduced amino acid sequence of
AsNODf32 with those of Cys proteinases of the papain super family. Dots
represent identical amino acid residues. Dashes represent blanks. Amino
acid residues (AsNODf32 numbering) involved in catalysis (C-149 and
H-286), active-site formation (Q-143, N-307, S-308, and W-309), and the
disulfide bridges (C-146/C-189, C-180/C-222, and C-280/C-322) are
underlined. A putative vacuole-targeting signal (LQDA) and the
ER-targeting signal (KDEL) are also indicated by dotted underlines,
respectively. As, Chinese milk vetch; Ag, A. glutinosa
(Goetting-Minesky and Mullin, 1994); Pm, P. menziesii
(Tranbarger and Misra, 1996); Ps, Pisum sativum (pea;
Kardailsky and Brewin, 1996); Pv, P. vulgaris (Sohlberg and
Sussex, 1997); Vm, Vigna mungo (Akasofu et al., 1989); Vs,
vetch (Becker et al., 1997).