Southern-blot analysis of Chinese milk vetch (A.s)
and M. huakuii (M.h) genomic DNAs. Restriction enzymes used
to digest DNA are as indicated. Probes used were a 148-bp N-terminal
fragment (A), a 453-bp C-terminal fragment (B), and an entire region
(1,291 bp, C) of AsNODf322 cDNA. Sequences used to generate probes A,
B, and C are illustrated in the bottom section, relative to a
restriction map of AsNODf32 cDNA. Arrows indicate the position of
lambda DNA fragments digested with HindIII.