Figure 5.

In situ hybridization analysis of the AsNODf32 gene expression. Median longitudinal sections of Chinese milk vetch nodules infected with M. huakuii (30 d post-infection) were hybridized with riboprobes containing digoxygenin. Hybridization signal was detected as a blue precipitate by staining with BCIP and NBT. A, Schematic representation of the five distinct regions (Vasse et al., 1990; Mylona et al., 1995) of an indeterminate type nodule of Chinese milk vetch. B, Nodule section hybridized with AsNODf32 antisense strand RNA probe. C, Nodule section hybridized with AsNODf32 sense strand RNA as the probe.