Figure 5.

Runx2 protein expression decreases in hDPSCs and increases in hBMSCs during odontoblast and osteoblast differentiation, respectively. The expression of Runx2 in (A) hDPSCs during odontoblast differentiation and (B) hBMSCs during osteoblast differentiation. Cells were incubated in mineralization medium for 0, 3, 5, 7, 14 or 21 days. Protein expression levels of Runx2 were determined using western blot analysis. Data are presented as the mean ± standard error of the mean of at least three experiments. *P<0.05 and **P<0.01 vs. 0 d. Runx2, runt-related transcription factor 2; d, day; hDPSCs, human dental pulp stem cells; hBMSCs, human bone marrow mesenchymal stem cells.