Figure 6.

Effects of Smad2 on Col I and Col III expression in hHSFs. hHSFs cells were transfected with Smad2-plasmid or Smad2-siRNA. Smad2, Col I and Col III expression were determined by (A) western blotting and (B-D) reverse transcription-quantitative polymerase chain reaction at 48 h post-transfection. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01 vs. Con-p; ^#P<0.05 and ^##P<0.01 vs. Con-s. Smad2, mothers against decapentaplegic homolog 2; Col, collagen; hHSFs, human hypertrophic scar fibroblasts; Smad2-siRNA, cells transfected with Smad2-small interfering RNA; Con-p, cells transfected with control plasmid; con-s, cells transfected with control siRNA; plasmid, cells transfected with Smad2-plasmid.