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. 2018 Mar 6;15(5):6947–6956. doi: 10.3892/ol.2018.8180

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Copyright: © Zhou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — USP1 was a direct target of miR-192-5p in osteosarcoma cells. (A) Bioinformatics study suggested that there was a putative binding site between miR-192-5p and USP1. Dual-luciferase reporter system assay indicated that miR-192-5p mimics significantly restrained wild-type 3′UTR-USP1 reporter activity, while there was no repression effect on the mutant 3′UTR-USP1 reporter activity in (B) 143B and (C) U2OS cells. (D) Ectopic expression of miR-192-5p decreased the USP1 mRNA expression in 143B and U2OS cells. (E) Overexpression of miR-192-5p also reduced the expression of USP1 at the protein level. Data are presented as the mean ± standard deviation of 3 independent assays. ***P<0.001 vs. miR-NC. miR, microRNA; NC, negative control; USP1, ubiquitin-specific protease 1; UTR, untranslated region; wt, wild type; mut, mutant.