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. 2018 Mar 6;15(5):6947–6956. doi: 10.3892/ol.2018.8180

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Copyright: © Zhou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — miR-192-5p regulated osteosarcoma cell proliferation, apoptosis, migration, invasion and chemo-sensitivity through USP1. (A) The USP1 mRNA expression was detected by reverse transcription-quantitative polymerase chain reaction. (B) The USP1 protein expression was detected by western blot analysis. (C-I) Ectopic expression of USP1 statistically enhanced cell (C and D) proliferation, decreased cell (E and F) chemo-sensitivity, promoted cell (G) migration and (H) invasion (magnification, ×100), and reduced cell (I) apoptosis in 143B and U2OS cells, which could be partially abolished by miR-192-5p following co-transfection with miR-192-5p mimics and USP1. Data are presented as the mean ± standard deviation of 3 independent assays. *P<0.05, **P<0.01 and ***P<0.001 vs. NC, or as indicated. miR-NC. miR, microRNA; NC, negative control; USP1, ubiquitin-specific protease 1; PI, propidium iodide.