Lentivirus-mediated overexpression of miR-124 suppresses gastric cancer cell growth, migration and invasion. (A and B) Cell Counting kit-8 assays showed that overexpression of miR-124 inhibited SGC-7901 (A) and AGS cells (B) growth. (C and D) Transwell assays revealing that overexpression of miR-124 inhibited SGC-7901 and AGS cells migration and invasion. Micrographs were captured at magnification, ×200. ***P<0.001 (vs. the negative control group). (E and F) Overexpression of miR-124 in gastric cancer cells inhibited the growth of xenografts in nude mice. SGC-7901 cells transduced with Lenti-miR-124 or Lenti-GFP were injected subcutaneously into the right back to establish xenograft model. At the final time point (37 days after the first injection), tumors in the two groups measured (E) in situ and (F) following resection were shown for the two groups. (G) Statistical analysis of the tumor volume in Lenti-miR-124 and Lenti-GFP groups revealed that overexpression of miR-124 significantly delayed tumor growth compared with the control group. miR-124, microRNA-124; Lenti-miR-124, lentiviral vector expressing miR-124; Lenti-GFP, lentiviral vector expressing miR-124 and green fluorescent protein. *P<0.05, **P<0.01, ***P<0.001.