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. 2018 Mar 7;15(5):7450–7458. doi: 10.3892/ol.2018.8194

Figure 3.

Figure 3.

Lentivirus-mediated overexpression of miR-124 repressed the expression of JAG1 and EZH2 in gastric cancer cell lines. (A and B) Western blot analysis revealed the expression levels of JAG1 and EZH2 in gastric cancer SGC-7901 (A) and AGS (B) cells transduced with Lenti-miR-124 or Lenti-GFP. (C) JAG1 mRNA levels were determined in 30 fresh GC tissues and matched adjacent non-tumor tissues using RT-qPCR. Paired t-test was used to analyze the difference between two groups. (D) EZH2 mRNA levels were determined by RT-qPCR in the same cases of fresh GC tissues and paired adjacent non-tumor tissues as previously used to detect JAG1 mRNA levels. Paired t-test was used to analyze the difference between the two groups. miR-124, microRNA-124; Lenti-miR-124, lentiviral vector expressing miR-124; Lenti-GFP, lentiviral vector expressing miR-124 and green fluorescent protein; JAG1, jagged1; EZH2, enhancer of zeste homolog 2; GC, gastric cancer; RT-qPCR, reverse transcription-quantitative polymerase chain reaction. *P<0.05, **P<0.01, ***P<0.001.