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. Author manuscript; available in PMC: 2018 Apr 27.
Published in final edited form as: Chemistry. 2015 Aug 18;21(39):13598–13608. doi: 10.1002/chem.201502211

Figure 7. Effects of allosteric ligands on yeast Hsp90 (Hsc82) interaction with the ATPase inhibitory co-chaperone Sba1.

Figure 7

The presence of allosteric accelerators minimizes AMP-PNP-dependent association of Sba1 (p23) with Hsc82. These data were obtained by adding compounds to yeast lysates followed by affinity pulldown of His-tagged Hsc82 with Ni-NTA agarose beads. Hsc82 and Sba1 were visualized in pulldowns and lysate with appropriate antibodies (see Experimental).