Figure 2.

Northern-blot analysis of POTLX-3 and Pin-2 mRNA accumulation after wounding potato leaves. Total RNA was isolated from wounded leaves of potato cv Superior plants harvested 0 to 72 h after wounding. The 0-h RNA sample was extracted from leaves harvested immediately after wounding. RNA samples (18 μg per lane) from wounded leaves were loaded on duplicate gels, and northern blots were probed with ³²P-labeled inserts of either POTLX-3 cDNA or proteinase inhibitor II (Pin-2) cDNA. Total RNA (18 μg per lane) from leaves treated with ethylene for 24 h was included as a positive control. Hybridization to the Pin2 probe was conducted as a positive control for wounding. POTLX-3 transcript size is approximately 2.8 kb. Equal loading of RNA samples into each lane was confirmed by visualizing the RNA gel stained with ethidium bromide.