Figure 3.

Northern-blot analysis of POTLX-3 mRNA accumulation in potato leaves treated with various phytohormones. Leaf-petiole cuttings were incubated for 6, 12, 24, and 48 h in either water (control), 100 μm ABA, 100 μm MJ, 100 μm gibberellic acid (GA₃), 50 μm auxin (NAA), or in hermetically sealed 4-L jars that contained 10 μL/L ethylene. An equal amount of total RNA (12 μg per lane) was loaded in each lane, and the consistency of loading was determined by visualizing RNA under UV light after staining with ethidium bromide. Hybridization was conducted with the ³²P-labeled 1.5-kb EcoRI fragment of the POTLX-3 cDNA clone. Blots subsequently were stripped and rehybridized with probe derived from a proteinase inhibitor II (Pin-2) gene.