Figure 6.

Northern-blot analysis of POTLX-3 mRNA accumulation in response to P. syringae pv phaseolicola inoculation. cv Superior leaflets were inoculated with P. syringae pv phaseolicola bacterial suspension (1.2 × 10⁸ colony forming units mL⁻¹) (A) or with sterile water (mock-inoculated) (B). Plants with inoculated leaves were incubated for 0, 3, 6, 12, 18, or 24 h in the greenhouse, and total RNA was extracted from the entire leaflet at each time. Blots of total RNA (18 μg per lane) were hybridized with the ³²P-labeled 1.5-kb EcoRI fragment of the POTLX-3 cDNA clone. Equal loading of RNA samples into each lane was confirmed by stripping and rehybridizing the blots with a 1.2-kb wheat 18S ribosomal RNA probe.