Figure 2.

WDR5 expression was significantly elevated in HCC cell lines. (A) Reverse transcription-quantitative polymerase chain reaction was used to analyze WDR5 expression in HCC cell lines and a normal liver cell line. (B) Western blotting was used to analyze WDR5 expression in HCC cell lines and a normal liver cell line. *P<0.05, **P<0.01 vs. HL7702. (C) shRNA transfection was used to downregulate WDR5 expression in HCC cell lines. **P<0.01 vs. untransfected group. Statistical analysis was performed with one-way analysis of variance and Tukey's test. WDR5, WD repeat domain 5; HCC, hepatocellular carcinoma; shRNA, short hairpin RNA; NS, not significant.