Figure 6.

Hypoxia regulates Snail protein through HIF-1α in pancreatic cancer cells. (A) Western blot analysis demonstrated that the hypoxia group had significantly higher Snail expression, compared with the normoxia and hyperoxia groups (*P<0.05). (B) Following treatment with the HIF-1α-specific inhibitor, YC-1, western blot analysis indicated that Snail protein expression did not differ among the different oxygen environments (P>0.05). (C) Immunofluorescence data demonstrated that Snail fluorescence intensity did not differ between cells exposed to normoxic, hyperoxic and hypoxic conditions following treatment with the HIF-1α-specific inhibitor, YC-1 (×400). Hypoxia, 5% oxygen; normoxia, 21% oxygen; hyperoxia, 30% oxygen; E-cadherin, epithelial-cadherin; HIF, hypoxia-inducible factor.