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. 2018 Mar 20;15(5):4191–4198. doi: 10.3892/etm.2018.5973

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Copyright: © Rong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — MTX inhibited TG-induced PC12 cell apoptosis. (A) MTT assay of PC12 cells treated with 1, 2.5, 5, and 10 µM TG. (B) MTX treatment significantly reversed the TG-induced decrease in cell viability by 46.7%. (C) Flow cytometry analysis of PC12 cells treated with 5 µM TG in the presence or absence of MTX. FL1-A on the X-axis represents the relative fluorescence intensity value; FL2-A on the Y-axis represents the relative fluorescence intensity value. Data are presented as the mean ± standard error of the mean. *P<0.05, **P<0.01 and ***P<0.001 vs. NC; ^#P<0.05. NC, normal control; MTX, methotrexate; TG, triglycerides; OD, optical density.