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. 2018 Mar 22;15(5):8079–8087. doi: 10.3892/ol.2018.8323

Figure 1.

Figure 1.

Synthesis and characterization of oxaliplatin-Au-Fe3O4-Herceptin NPs. (A) Transmission electron microscope images of dumbbell-shaped structure of 8–20 nm Au-Fe3O4 NPs, formed of 8 nm Au NPs (black) and 20 nm Fe3O4 NPs (grey). (B) Mass spectrometry analysis of Au-Fe3O4-Herceptin NPs. A specific peak for human epithelial growth factor receptor antibody, Herceptin (Mr=148), at m/z ~148,000 was detected by matrix-assisted laser-desorption ionization-TOF-TOF. (C) Infrared spectrum of Au-Fe3O4-Herceptin NPs. Specific double peaks of carbonyl bands at 1580 and 1650 cm−1 (arrow) representing the amide bonds linking the polyethylene glycol to the silane. (D) NMR of the oxaliplatin-binding ligand (D2O, 300 MHz) revealed peaks at δ 3.51 (8.00 H, s, 3-H), 2.97–3.02 (3.80 H, t, 2-H) and 2.72–2.76 (3.72 H, t, 1-H). (E) The NMR analog of oxaliplatin-binding ligand. (F) The released oxaliplatin level from pH=6.0 group was significantly increased compared with pH=8.0 group. (G) The released oxaliplatin level at pH=7.3 after 4 h of incubation was significantly compared with the free oxaliplatin group. *P<0.01.*P<0.01 vs. pH=8.0. NMR, Nuclear Magnetic Resonance; NPs, nanoparticles; TOF, time of flight; Pt, oxiplatin.