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Operating conditions of MALDI-TOF MS.
| Parameters | Settings |
|---|---|
| Mode | Reflectron positive-ion mode |
| Laser | 200 Hz smartbeam-I laser |
| Delay | 80 ns |
| Ion source | 1 voltage, 25 kV; 2 voltage, 23.4 kV |
| Lens voltage | 6 kV |
| Mass range | 2,000–20,000 kDa |
| Software | FlexImaging 3.0 software (Bruker-Michrom, Inc.) |
