Figure 4.

Effects of inhibitors of protein phosphatases and protein kinases on the expression of din genes. A, Cells were pre-incubated for 1 h with 1 μm okadaic acid (OKA), 4 μm K-252a (K252), or 75 μm genistein (GEN). Cells were rinsed and incubated with each inhibitor for 12 h in a Suc-free (−) or a Suc containing (+) medium. Cells were also incubated for 12 h in the absence of inhibitors (CTR). B, Cells were incubated with 1 μm of calyculin A (CA) for 12 h, either in a Suc-free (−) or a Suc-containing (+) medium. Each lane was loaded with 10 μg of RNA.