Figure 5.

Effects of protein phosphatase inhibitors on the relative mRNA levels of din genes. Relative levels of the transcripts were estimated by quantitation of signals on the blotted membranes (Fig. 4), with a Bio Imaging Analyzer BAS2000 (Fuji Photo Film, Tokyo). The results were expressed as a percentage of the respective maximum level for each gene. Cells were incubated in a Suc-containing (white bars) or a Suc-free (shaded bars) medium for 12 h in the absence of inhibitors. For the treatment of inhibitors, cells were incubated with okadaic acid (A) or calyculin A (B) for 12 h in a Suc-containing (hatched bars) or a Suc-free (black bars) medium.