Fig. 4.

Bortezomib reduces TRAF6 protein expression through autophagy-mediated lysosomal degradation. a Western blot analysis of TRAF6 protein expression in SAS cells. Cells were treated with 6 Gy of IR or 25 nM of bortezomib or in combination for 12, 18 and 24 h. b Western blot analysis of TRAF6 protein expression in SAS cells. The cells were treated with bortezomib alone with 10, 15, 20, 25, or 30 nM of bortezomib for 24 h. c Western blot analysis of TRAF6 protein expression in SAS cells. Cells were treated with 6 Gy of IR or 25 nM of bortezomib or 1 μM of MG132 or in combination for 24 h. d Western blot analysis of TRAF6 protein expression in the absence or presence of 3-MA. Cells were pretreated with 3-MA (2 mM) for 1 h before combined treatment (6 Gy of IR and 25 nM of bortezomib) for 24 h. e TRAF6 mRNA expression levels were measured by real-time RT-PCR in the indicated cell lines and SAS cells treated with 6 Gy of IR or 25 nM of bortezomib alone or in combination for 24 h