Fig. 3.

Auxin signaling genes are induced by root cutting. (A–C) Relative expression of IAA19, ARF19 and LBD29 after root cutting. Error bars indicate the SE of three independent biological replicates. (D) The expression pattern of IAA19. The roots of 6-day-old seedlings expressing pIAA19::GUS were cut at 12 mm from the root–shoot junction. After 6 h, β-glucuronidase (GUS) staining was performed. The expression of pIAA19::GUS was observed in the cut end of root-cut plants and the corresponding area in intact plants. Scale bar = 0.1 mm.