Fig. 6.

The role of YUCCA9 (YUC9) following root cutting. Four-day-old WT (A) and yuc9 (B) plants were transferred to medium with or without NPA and incubated for 1 d before root cutting. Photographs were taken 4 d after root cutting. Scale bars = 1 cm. Red arrowheads indicate the point 12 mm from the root–shoot junction that corresponds to the cut point. (C) LR number of the WT and yuc9 following root cutting in the presence or absence of NPA. LR number was counted within 12 mm from the root–shoot junction 4 d after root cutting. (D) Relative expression level of YUC9 after root cutting. (E) IAA level of the WT and yuc9 at the indicated time points. (F) Progress of LR development after root cutting. The roots of 5-day-old plants were cut at 0 h. The number of LRPs or LRs was counted and the sum of LRP and LR number was defined as the total. (G) LR number of msg2-1 and msg2-1 yuc9 following root cutting in the presence or absence of NPA. LR number was counted within 12 mm from the root–shoot junction 4 d after root cutting. Error bars indicate the SE from 16 seedlings (C, F, G) or from three independent biological replicates (D, E). *Significant differences compared with WT plants (C, F), msg2-1 (G) or 0 h (E) (Student’s t-test, P < 0.05).