TABLE 1:
Primers used to generate the constructs used in this paper.
| Primer name | DNA sequence | 5’ RE site |
|---|---|---|
| SS-EGFP-F | GTGGCTAGCGTGAGCAAGGGCGAGGAG | NheI |
| SS-EGFP-R | GACTGACTGAATTCCTACTTGTACAGCTCGTCCATG | EcoRI |
| SS-EGFP2-F | GCTGTACAAGGGGCACGGGACCGGGTCTACAGGGAGCGGGAGCGTGAGCAAGGGCGAGG | BsrGI |
| SS-EGFP2-R | AACGGGCCCGGCTGCCAATCATGACTGTTACTTATACAGCTCGTCCATGCCG | ApaI |
| SS-EGFP-SUN1457–913-F | GCTGTACAAGGGGCACGGGACCGGGTCTACAGGGAGCGGGAGGGTGGACGATTCCAAGG | BsrGI |
| SS-EGFP-SUN1457–913-R | GAATTCCTACTGGATGGGCTCTCCG | EcoRI |
| SS-EGFP-SUN1702–913-F | ACATCCGAGGCTATTGTGTC | — |
| SS-EGFP-SUN1777–913-F | TGGTACTTCTCACAGTCACC | — |
| SS-EGFP-SUN2261–731-F | GCTGTACAAGGGGCACGGGACCGGGTCTACAGGGAGCGGGTCCTGGTGGGCAGCAAAAG | BsrGI |
| SS-EGFP-SUN2261–731-R | TTTTGAATTCCTAGTGGGCAGGCTCTC | EcoRI |
| SS-EGFP-SUN2520–731-F | TTGGTGAGCCGCCGC | — |
| SS-EGFP-SUN2595–731-F | TGGTACCACTCCCAGTCAC | — |
| SS-EGFP-SUNΔ-R | CCCGCTCCCTGTAGACC | — |
| SSΔ-F | GTGGCTAGCGTGAGCAAGGG | — |
| SSΔ-R | CATGGATCCGAGCTCGGTACC | — |
The F or R in the primer name refers to forward or reverse, respectively. Restriction enzyme (RE) cut sites are underlined. The sequence encoding the linker is bolded.