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. 2018 May 1;29(9):1012–1020. doi: 10.1091/mbc.E17-07-0466

FIGURE 4:

FIGURE 4:

Glucose regulates Bul1 phosphorylation. (A) Western blot from total protein extracts prepared from cells expressing ether Bul1-Flag or Bul1(PYm)-Flag and grown as indicated. (B) Line scan of the Western blot presented in A showing pixel intensity (relative to the max intensity for each lane) over 100 pixels. (C) Western blot on total protein extracts prepared from cells expressing ether Bul1-Flag or Bul1(PYm)-Flag and grown as indicated, and treated or not with calf intestinal phosphatase (CIP). (D) Line scan of the Western blot presented in C.