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. 2018 May 1;29(9):1137–1152. doi: 10.1091/mbc.E17-08-0515

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© 2018 Cestari et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — RBPs associated with stage specific gene regulation. (A) Differential expression of RBPs after knockdown of IPMK (36 h) or other IP pathway genes (24 h). (B) Real-time PCR analysis of 10 selected RBPs after 24 and 36 h IPMK knockdown. (C) Real-time PCR analysis of 44 genes from BF and PF gene sets after tet-regulatable overexpression of C-terminally V5-tagged RBPs in T. brucei BFs cultured in HMI-9 at 37°C for 24 h or SDM-79 at 27°C for 36 h. (D) Flow cytometry analysis of EP procyclin expression in T. brucei BFs overexpressing RBP6-V5 or PPCT1-V5. Cells were incubated in SDM-79 at 27°C for 3 d. (E) Flow cytometry quantification of EP procyclin expression after RBPs overexpression in T. brucei BFs (as described in D). Tet, 1 µg/ml. Data are represented as mean (heat maps) or mean ± SEM (bar graphs).