FIGURE 7:

IPMK regulates oxidative phosphorylation inhibitor sensitive ATP production in BFs. (A) RNAseq analysis of metabolic genes after knockdown of IPMK (36 h) or other IP pathway genes (24 h). RNAseq data of knockdown (tet-) cells were compared with data on their respective nonknockdown (tet+) cells. (B) Measurements of ATP produced per cell after 48 h IPMK knockdown in absence or presence of oxidative phosphorylation inhibitors. Antimycin (8 mM), potassium cyanide (K cyanide, 50 µM), sodium azide (0.5 mM), rotenone (5 µM), oligomycin (50 ng/ml), atractyloside (200 µM), and SHAM (100 µM) were added to the culture after 24 h IPMK knockdown. Tet, 0.5 µg/ml. Data are represented as means. *p < 0.05; **p < 0.01, ***p < 0.005 comparing tet- conditions treated with inhibitors vs. tet- cells not treated (No inhibitor) by t test.