TABLE 1:
Kinetic parameters of WT and mutant T. brucei rIPMK.
| Wild type | D142A | K164W | D142A/K144A | |
|---|---|---|---|---|
| Ins(1,4,5)P3 | ||||
| Km (µM) | 61.41 (±15.37) | 113.0 (±31.93) | 51.04 (±13.84) | ND |
| Vmax (nmol/min per µg) | 617.0 (±62.64) | 660.2 (±92.97) | 629.6 (±65.16) | ND |
| Kcat/Km (S–1M–1) | 1.67 × 105 | 9.73 × 104 | 2.06 × 105 | ND |
| Ins(1,3,4,5)P4 | ||||
| Km (µM) | 91.44 (±52.01) | 153.5 (±33.97) | 169.9 (±20.39) | ND |
| Vmax (nmol/min per µg) | 400.8 (±52.01) | 315.2 (±38.48) | 540.3 (±36.96) | ND |
| Kcat/Km (S–1M–1) | 7.31 × 104 | 3.42 × 104 | 5.23 × 104 | ND |
ND, not determined. The D142A/K144A mutation eliminates enzyme activity.