Fig. 3.

Hypoxia-induced miR-93 was upregulated in PCa with prognostic significance, while TGFBR2 was a bona fide direct target of miR-93. a. Compared to cells in normoxia, PCa cells in hypoxic conditions showed markedly increased expression of miR-93. b. Induction of miR-93 was attenuated under hypoxia in PCa cells after treatment of siRNA for HIF-1α. c. miR-93 expression levels are significantly elevated in PCa tissues in comparison to normal ones. d. Compared to normal prostate epithelial cell RWPE-1, PCa cell lines showed significant higher expression of miR-93. e. miR-93 expression levels were significantly increased in patients with Gleason score (GS) > 7 in comparison to the patients with GS ≤ 7. f. miR-93 expression levels were significantly increased in patients with higher tumor stage in comparison to the patients with lower one. g. Significant negative correlation between the expression of miR-93 (MIMAT0000093) and TGFBR2 in TCGA data. h-i. Both mRNA (H) and protein expression (I) of TGFBR2 were significantly decreased after transfection of miR-93 mimics, while TGFBR2 expression elevated after transfection of miR-93 inhibitor. j. Respective figure of seed sequence of TGFBR2 via TargetScan. k. Dual luciferase assay validated that miR-93 mimics silenced the wide-type TGFBR2 while had no inhibitory effect on seed sequence mutated TGFBR2. *, P < 0.01; **, P < 0.01