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. Author manuscript; available in PMC: 2018 Apr 27.
Published in final edited form as: React Oxyg Species (Apex). 2016;1(2):157–164. doi: 10.20455/ros.2016.819

FIGURE 2. Linear relationship between B16-F10 melanoma cell number and the bioluminescence intensity.

FIGURE 2

Bioluminescence was measured at 25.6°C for 5 min after adding samples derived from 1–160,000 B16-F10-luc-G5 cells (without zeocin selection) to the reaction mix containing 100 μg/ml of luciferin and 0.5 mM ATP. Data represent means from 4 separate experiments with the unit of bioluminescence being total counts of photon emission over 5 min.